Humana Press

Humana Press

English

"It contains cutting-edge molecular diagnostic techniques with detailed step-by-step instructions and tips to obtain successful results....the purpose here is to offer readily reproducible molecular pathology techniques that are either frequently performed or recognized for their significant diagnostic utility. It is a worthy objective and the editor meets it very well....Certain methodological details are described in the notes, which provide much helpful information in methodology and tips on how to avoid pitfalls. This form of presentation is attractive both to newcomers to the field and to those with some experience in diagnostic molecular pathology....Cutting-edge methods include telomerase, microsatellite instability, chromosomal translocations, and fluorescent in situ hybridization assays....The significant diagnostic utility of molecular protocols in cancer, hermatological malignancies, and virus related diseases is covered well....It is a good protocol resource book in molecular pathology."-Doody's Health Sciences Book Review Journal "Molecular Pathology Protocols fills a unique niche for general molecular pathology laboratories that may be called upon to perform a wide variety of tests for infectious and noninfectious diseases. It has 35 chapters that provide extensive detail on laboratory protocols for a variety of important conditions, from techniques for preparation of nucleic acid templates and mutation detection for genetic and neoplastic evaluations, to hematological applications, to a limited number of detailed protocols for infectious disease diagnosis and monitoring...The major strength of the protocols discussed in this book lies in their enormous detail and the practical remarks of the authors, which will assist in establishing the assays in a new laboratory. Another great value in some chapters is the emphasis on the assessment of quality control that will meet or exceed all the requirements of agencies that license and regulate clinical laboratories." - Clinical Infectious Diseases "A must for genetic laboratories and all those using the new molecular biological methods." - Journal of Endocrine Genetics "Each protocol in this book includes sections of introduction, materials, methods, and notes. Certain methodological details are described in the notes, which provide much helpful information in methodology and tips on how to avoid pitfalls. This form of presentation is attractive both to newcomers to the field and to those with some experience in diagnostic molecular pathology. Most protocols use polymerase chain reaction (PCR) based techniques. Cutting-edge methods include telomerase, microsatellite instability, chromosomal translocations, and fluorescent in sity hybridization assays. This book has good references in both background of diseases and techniques. Most charts and labels have high quality;...The significant diagnostic utility of molecular protocols in cancer, hematological malignancies, and virus related diseases is covered well....This book is well written and the molecular protocols introduced can be easily followed practically." - E-Streams